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Image Search Results
Journal: The Journal of biological chemistry
Article Title: Activation transcription factor 1 involvement in the regulation of murine H-2Dd expression.
doi: 10.1074/jbc.272.25.15993
Figure Lengend Snippet: FIG. 4. Gel supershift assay with anti-ATF-1, anti-c-Jun, and anti-ATF-2 antibodies. Nuclear extracts (1 mg) from the RadLV- induced, thymoma-derived cell line were incubated with end-labeled, double-stranded oligonucleotide (1 3 105 cpm) containing H-2 BF1 target sequences. Lane 1 had no antiserum added. Specific antibodies against ATF-1 (lane 2, sc-270x; lane 3, sc-243x), c-Jun (lane 4, sc-45x), and ATF-2 (lane 5, sc-187x) were mixed with parallel binding reactions, incubated for 1 h on ice, and loaded onto gel. All antibodies were purchased from Santa Cruz Biotechnology. Supershifts of the H-2 BF1 complex with anti-ATF-1 antibodies were observed: an asterisk (*) in lane 2 and double asterisks in lane 3.
Article Snippet: Specific
Techniques: Derivative Assay, Incubation, Labeling, Binding Assay